I guess the simplest method is just to measure several lines across the particle and take an average? You haven't provided an image so it is incredibly difficult to understand how irregular your particle is.

For the area you can greyscale and threshold, see: https://helloacm.com/how-to-compute-the-area-of-irregular-shapes-using-imagej/

You could also use the Feret's diameter, see:

https://imagej.nih.gov/ij/docs/menus/analyze.html#set

Or ParticleSizer:

http://imagej.net/ParticleSizer

Kind regards,

Jules

If all the particles have similar irregular shape, you should measure the largest diameter across each particle and then take the average value.

Otherwise, measure several diameters for each particle and take average for each. ParticleSizer tool is another good option.

This would also be morphology dependent, what is the morphology of the nanostructures? Please also provide us with information about the estimate sizes, sometimes TEM is better suited for accurate particle size analysis.

Hi Deepak

A common method for denoting the size of a non spherical particle is to report the Equivalent Circle Diameter, which is the diameter of a circle with the same area.

I believe that this is a parameter that can be reported from the inbuilt analysis in ImageJ.

To add to the complication of image interpretation, remember that your image is a 2D representation of a 3D object.

please check these link

https://www.youtube.com/watch?v=JQAZ--c9YfI&t=139s

https://www.youtube.com/watch?v=FiFwxoxOmNo

https://www.youtube.com/watch?v=FiFwxoxOmNo

Thank you all,

@Alexander Malm

Same concept i was thinking. Is there any reference paper doing as the same?

Hello

Use SPIP software, Its good for irregular shape particle .

https://www.imagemet.com/products/spip/

you can used the daimeter averge of several particals , also you can deal that by drow the circles around the partical shapes.

please follow this vedio (https://www.youtube.com/watch?v=JQAZ--c9YfI&t=139s)

also , try to use the programs of that

http://imagej.net/ParticleSizer

https://helloacm.com/how-to-compute-the-area-of-irregular-shapes-using-imagej/

Thankyou sir.. i did the same

According to me, you should also tally your results obtained from Image J with analysis results of the particle size analyzer.

You just see in the you tube.

You can find the solution here

https://www.youtube.com/watch?v=2BLRYrH3b4I

or manually measure the size ofthe particle with line tool and use (Ctrl+M) for measuring the corresponding data then simply plot it with Origin and the procedure will be there in youtube ...

Thanks

I would suggest you measure the largest diameter across each particle and then take the total average value.

This need to mention in your methodology to measure the particle size.

Here is a reference for the equivalent circle diameter as requested:

Pabst, W. & Gregorova, E. Characterization of particles and particle systems. ICT Prague 2007 1–122 (2007).