Dear Nimesha,

It is possible to pre-treat the water sample and add a nitrite specific chromophore to it, then measure the visible Absorbance, using a visible spectrophotometer.

Alternatively, nitrite levels may be determined by the use of anion ion chromatography. Please see the attached application note.

Kind regards,

Ade Kujore

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I have the measurement protocol but in French you need to translate it

it is attached

Hello Nimesha,

Measure out 50 ml of the water sample; add 1 ml of NEDA (

N-(1-Naphthyl)ethylenediamine dihydrochloride) to it. Wait for couple of minutes and then add equal volume of Sulfanilamide to it. Through diazotization reaction a complex compound forms rendering the mixture brilliant pink in color. This can be measured using a spectrophotometer at 540 nm in terms of its absorbance. The absorbance is the function of the intensity of the colour which is the direct result of the nitrites present in the water. You can proceed in the same manner for determining nitrate concentration in water sample but the water has to pass through a copperised cadmium containing reduction column so that the nitrate can be reduced to nitrite.Keep in mind that all these methods are for the estimation of dissolved inorganic nitrogen oxides. The absorbance values can be multiplied with the factor obtained from the slope of the curve generated through the use of standard nitrite solution gradients of known concentration.

Use of ion chromatograph as mentioned by Ade Kujore is also very useful method for determination of nitrite-nitrogen.

Regards,

Dr. Abhishek Mukherjee

Dear Ade Kujore , (Ms.) Fatima Zahra and Dr.( Mr.) Abhishek Mukherjee ,

Thank you for your kind response and valuable suggestions.

Best Regards,

Nimesha.

Hi Nimesha,

in our lab we use the method proposed by Strickland & Parson 1968. We've been using it for quite a while, and it always proved to be a good way of measuring Nitrite in water.

Filter 50ml of sample water through whatman filter paper no 1. Add 1ml of sulphinamide reagent (1gm in 100 my of 10% HCL). After 3-5 minutes add NEDA to it. In acid solution the nitrite yields nitrous acid which diazotises sulphinamide. The diazonium salt reacts with aromatic NEDA forming a pink azo dye which is spectrophotometrically determined at 543nm. Deduce the nitrite from a standard curve. Standard curve can be prepared taking sodium nitrite of known concentrations.

You can also try the Aminot et Chaussepied (1983) methode, it's easy and have all details for the measurement you need. these are the chemical products used in this measurement:

- Concentrated HCl -Sulfanilamide ( C6H8N2O2S ) - N- (1-Naphtyl) ethylenediamine dihydrochloride -NaNO2 anhydrous -Chloroform You will need also a spectrophotometer.

Dear Mr.Pedro, (Ms.)Susiri and (Ms)Saad,

Thank you for your kind response and valuable suggestions.

Best Regards,

Nimesha.

Hi Nimesha

Try to use Rodier Method (Water analysis: wrote by Jean Rodier 9th edition)

Take 50 ml of water to be analyzed, - Add 1 ml of the mixed reagent. Wait 10 minutes. The appearance of the pink color indicates the presence of NO2-. Perform the reading by placing the water sample in a spectrophotometer at a wavelength of 543 nm. The result is given directly in mg / l.

Hello Nimesha,

Try to get standard ISO 6777:1984, "Water quality - Determination of nitrite - Molecular absorption spectrometric method".

Perhaps, you can get it through researchgate.net:

Article Water quality. Determination of nitrite. Molecular absorptio...

Best wishes,

Obrad

use device called photometer 7100, take 10ml of the sample in cup and add NO2 detector and stirred until it melts, take 10ml of the same sample for weight of device without any detector. Leave for 20minutes and then read directly from the device and multiple 3,3 or more fixed constant.

Dear All,

Thank you for your kind response and valuable suggestions.

Best Regards,

Nimesha.

dear Nimesha

you can use spectrometer method.

-best