Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

How can I get the coding gene sequences of Hela and U2OS cell lines? Is mRNA sequence of Hsp90 alpha tissue specific??

I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...

22 February 2021 1,853 3 View

What is the difference between actin and loading control in normalization of western blot???

Regarding normalization of western blot analysis, I have found different strategies people follow but I couldn't understand the principle and which one is the accurate, 1- some refer to loading...

04 December 2020 5,721 8 View

Detecting bands by ImageJ on mac not fixed properly in each lane???

I am using ImageJ on mac and when I select bands, the first one detected correctly, when I click cmd 2 (= ctr 2 on windows system) to detect the second band, the rectangle gets fixed on the...

01 December 2020 9,538 4 View

My question is about how to determined MIC concentration in the use of microdilution method?

I would like to ask you about MIC first, after I searching in the internet about my query I could not found any answer. My question is about how can I calculate the concentration of stock...

23 November 2020 6,737 3 View

Can you please help me with organic acid HPLC analysis?

Hello everyone, I am experiencing difficulties in the HPLC analysis of adipic acid. I have tried Atlantis C18, mobile phase phosphoric acid solution PH 2.65, flow rate 0.7 ml/min, column temp 35...

20 November 2020 8,280 4 View

Is THC causing underlying paranoia in individuals?

In other words, is there a chemical impact caused by THC which could be activating neural correlates in the insular cortex (ic) of the brain? The increase in the sensory activating...

04 August 2020 967 4 View

Is vitamin C beneficial against Corona virus?

Vitamin C (ascorbic acid) is a water-soluble vitamin that is thought to have beneficial effects in patients with severe and critical illnesses. It is an antioxidant and free radical scavenger that...

04 August 2020 3,856 98 View

How long does the coronavirus stay on your clothes?

03 August 2020 1,674 96 View

Are health workers at risk from a novel coronavirus?

Are health workers at risk from a novel coronavirus? Yes, they can be, as health care workers come into contact with patients more often than the general public WHO recommends that health care...

27 July 2020 1,027 97 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

What are the most relevant MSc proposal topics for Petroleum Production Engineering at the moment?

I am a Petroleum Production Engg MSc student at Robert Gordon University in Aberdeen and I am in the process of selecting a topic for my MSc Engineering Investigation proposal. I would very much...

01 March 2021 7,396 4 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

Covid 19 Pandemic ' impact on digital transformation?

Covid 19 Pandemic and digital transformation

22 February 2021 3,351 5 View

How can we improve AI in detecting fraudulent activities on digital payments?

I am conducting a study on the effectiveness of AI towards detecting and averting digital payment fraud. What are the popular AI techniques being adopted in mitigating digital fraud and how could...

21 February 2021 3,888 4 View

Are we using now IT technology effectively?

I discover now two my papers, which are more then 30 years old and which are describing something, what was done in years 1985 -...

21 February 2021 5,008 3 View