I am preparing liposome with hydrophilic drug. my problem is how to measure the amount of the drug that encapsulating in liposome and how to separate from the aqueous solution? as you know if i use centrifuge the liposome will break and that way we can't separate the drug that were encapsulated then that were in solution. in same time if i use filtration also, liposome will be break when hit the solid surface and lead to the same direction. so, what would you suggested me to do ?
Hi Asim,
The gentler way to "clean" the outer buffer of your liposome suspension is dialysis.
make sure to have the same osmomolarity between the dialysis buffer and your drug solution.
there is plenty of dialysis system available i usually use floatalyzer from Sigma Aldrich.
Another way is to use size exclusion chromatography (de-saltling column) but it yield in a small dilution of your sample.
hope it helped
Regards
Etienne
first you need to determine the osmomolarity of your drug buffer (using an osmometer if you don't have one you can do a "rough calculation")
the preparation of the dialysis tube depend on the manufacturer (follow their instruction)
place your liposome sample in the tube and place the tube in a buffer with the same osmomolarity (but without the drug)
i usually go for volume ration tube/becher of 1/100
buffer change depend on your system but a rule of thumb is change the buffer after 2h, 6h, 12h and your drug should be remove within 24h.
that's it
Regards,
Etienne
Two methods are described in the attached papers.
Article Microscopical investigations of nisin-loaded nanoliposomes p...
Article Formulation and characterization of nanoliposomal 5-fluorour...
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