Hello,

You may please refer to the review article below for alternatives. I hope it helps.

https://doi.org/10.1016/j.biochi.2005.01.007

PMID :15781313

Best.

Hi Rosemari Otton . Most MMPs are secreted as zymogens with little activity. To measure activities in solution you need to activate them with APMA (4-aminophenylmercuric acetate).

If zymography doesn't work for you, you could try quenched gelatin. It is FITC hyperlabeled gelatin that is non-fluorescent in solution, but becomes fluorescent when it is cleaved. Just be aware that most proteinases can also cleave this substrate.

Thermo DQ™ Gelatin From Pig Skin, Fluorescein Conjugate

Dera Rosemari, many thanks for sharing this very interesting technical question with the RG community. A detailed alternative protocol for measuring MMP activity using a fluorogenic substrate is described in the following potentially useful article:

Measuring Matrix Metalloproteinase Activity in Macrophages and Polymorphonuclear Leukocytes

Article Measuring Matrix Metalloproteinase Activity in Macrophages a...

Unfortunately this paper has not been posted as public full text on RG. However, the senior researcher of this study has an RG profile: https://www.researchgate.net/profile/Markus-Brown

Thus there is a good chance that you can request the full text directly from this author. I hope this information is helpful. Good luck with your research and best wishes, Frank Edelmann

Thank you very mu.ch for your attention and kind response. I´ll try to download the paper as you suggested. My best regards Frank T. Edelmann