Micron size particle. Need to understand its hardness for pump design
Hello everyone, I am currently working on a protein that is 10x his-tagged and positively charged (predicted pI=10.16). But when I tried to use Ni-NTA column to purify the protein, it's not...
24 July 2024 7,293 4 View
Hello, I am a master's student currently conducting lentivirus transduction experiments in Jurkat cells. The virus is not effectively entering the Jurkat cells, so I am planning to use an...
21 July 2024 4,676 4 View
My negative control cells had some damage, they are neoplastic cells and my anaplastic cell had even more damage in the negative control. Ok, but my question is, is there much difference between...
27 May 2024 7,529 2 View
I tested DNA structure (5uM dissolved in 50 mM Tris) using JASCO-1500 but found no peak at wavelength 220-350. The results look like a wave shape throughout the wavelength...
14 April 2024 4,003 2 View
Hi: I am performing immunofluorescence staining on 100 um 4%PFA fixed pancreas sections using our self-made polyclonal primary antibody. Tissue was gradient dehydrated in methanol diluted in 0.2%...
28 January 2024 867 3 View
I am conducting research on the effects of residential environments on mental, physical, and social health. In my questionnaire, mental, physical, and social health are measured by three separate...
10 January 2024 3,646 2 View
We used mouse cell line HEI-OC1 and checked OCTN1 expression by WB, then we did IP cell lysate with anti OCTN1 antibody and IB with OCTN1 antibody, surprising! The band is way above IB with WB (30...
04 January 2024 6,926 0 View
17th refers to the experimental parameter code Material is mesoporous silica nanoparticles (MSN-NH2). Use deionized water as dispersant and ultrasonic vibration. The theoretical concentration is...
02 January 2024 4,160 3 View
Forgive me if I may not have been standard in my formulation of the question. I wish to analyze the effect of residential environmental factors on depression through linear regression. In the...
05 December 2023 5,351 2 View
In our study, each participant rated several organizations randomly selected from a big pool on some attributes (denoted as) x1, x2, and x3 and an outcome y, and each organization has multiple...
14 November 2023 2,756 5 View
average particle size calculation from TEM
04 August 2024 2,921 1 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
The delta function seems produce logical contradictions when analyzed on a fundamental level. I would be curious if anyone else agrees.
31 July 2024 10,109 3 View
Some of the techniques I am familiar with are standard. I would like to know which of the two methods under study is preferable, as well as the cost and list of particles and devices needed in...
22 July 2024 1,921 3 View
Hi everyone, I experienced some moving particles in my mouse hippocampal slices. The media looks clear. Any idea what these moving particles might be? Thanks you!
22 July 2024 3,734 0 View
I make polycrystalline diamond with tape casting followed by HPHT sintering. When i sintered the samples up till May, the sintering was good. When i sintered the samples in June and July at the...
16 July 2024 3,212 5 View
anyone looking for research collaboration on the anaerobic digestion slurry and digestate can contact me for further details. As my whatsapp number is +91 8317585217
11 July 2024 6,753 3 View
Is any specific sample preparation required to perform VSM measurement of NdFeB alloy powder to get a square-shaped M-H loop? Or is it mandatory to make magnetically aligned and sintered pellets...
09 July 2024 1,969 0 View
I tried to make slurry for NCM cathode but there was problem in coating process. The detail of my experiment is following; The ratio between active material(NCM811), conductive additives(Super-P)...
08 July 2024 7,327 4 View
