Hi, I am Joe and currently, I am working in a neuroscience lab and focusing on the adrenergic beta and alpha receptors. And I have some technical problems with that, which in my experiment there is always low efficiency to stain the alpha 1and beta 2 receptor in mice brain. I have already tried several antibodies including the Almo, Bio, thermo antibodies
Therefore I would like to ask if there are any tips to increase the staining efficiency and do you guys have any recommendations of the antibodies for the Beta 2 and alpha1 receptor for mice? Thank you so much for your help.
My ex-colleague used this trick to maximize binding of primary antibodies to a target, which presented in a very scarce amount - she just simply incubated slides at +2 - 8*C overnight. But I don't remember if it was FFPE samples or "wet" slices from cryotome.
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