Hello,

I am currently working on transfecting Hela cells using the Neon NxT Electroporation System and I was wondering how to maximize my plasmid library.

This library is very precious and I would prefer to do transfections with as little DNA as possible while maintaining high transfection efficiency. The plasmid library I'm using is >10kb in size and I have about 200ug in total.

Any tips on what protocols you use would be greatly appreciated!