Kirby Fawcett

3 Questions 1 Answers 0 Followers

Questions related from Kirby Fawcett

How to maximize plasmid library for Hela cell transfection?

Hello, I am currently working on transfecting Hela cells using the Neon NxT Electroporation System and I was wondering how to maximize my plasmid library. This library is very precious and I...

02 April 2025 4,448 0 View

How many Hela cells should I seed per well of a 6 well plate to reach confluency within 1 week?

Anything helps!

09 January 2025 2,476 1 View

Is it okay to dissolve Ampicillin Anhydrous in 1 M NaOH?

So I made a 100 mg/ml solution of Ampicillin and in order to solubilize the powder I used 1 M NaOH. I noticed the solution turns yellow shortly after dissolving and I was wondering if some...

06 June 2024 7,239 0 View