Hi Yousaf,

you can dilute your 5 mg/ml stock solution directly in the medium used for the MIC assay. It should not precipitate as you go down a bit with the concentration. Anyway you can check it before doing the actual assay. If needed you could also do a pre-dilution in 50% DMSO and then go in your medium.

Hope that helped,

Francesco

Hi Yousaf,

I agree with Francesco. I would simply suggest you to dilute your stock solution directly in the medium, since DMSO is toxic per se, and it could hide the real MIC. Similarly, it would be useful if you could prepare a more concentrated stock dilution, so that you can serially dilute it, preferentially with the testing medium...

Anyway, take care to the pH of the solution, it could lead molecule precipitation even if it is dissolved in DMSO... but you should easily notice it at a glance.

cheers,

Irene

Thank You  Francesco and Irene.