step wise procdure in detail for preparation of ph senstive peptidal nano hydrogel
My formulation is Self assembly peptide hydrogel My hydrogel is Ph senstive,
07 August 2018 3,812 0 View
MAX 1 VKVKVKVK-VDPPT-KVKVKVKV-NH2 I am preparing Polypeptide nano hydrogel for this now i want to change sequence of MAX1 . How i can change sequence for MAX1? My second question is that i want...
02 March 2018 1,010 1 View
We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...
08 August 2024 4,842 0 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Is anyone here using the Thermo Fisher Nano Orange kit for protein quantification, and detecting the fluorescent signal on the Promega Quantus? We are struggling to make sense of the standard...
05 August 2024 8,946 3 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?
31 July 2024 6,544 11 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
My final research is about making chitosan/pva hydrogel for wound healing using gelatin as crosslinker. Last month, i made the thin film hydrogel and it turn well. It was okay. Last week, i make...
28 July 2024 2,794 6 View
I'm working with a hydrogel sample and I'd like to perform XRD analysis. Can anyone offer guidance on preparing the hydrogel for the XRD instrument? Specifically, I'm unsure about the best method...
20 July 2024 3,611 4 View
So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...
18 July 2024 4,075 3 View
Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...
10 July 2024 6,830 1 View