Dear Jyoti Jaglan

Maintaining a pure culture in full-scale treatment plants can be challenging due to less control over the experiments. However, using extremophiles such as thermophiles, halophile etc could be advantageous in that case.

Thank you

Chandan

My website: https://www.chandanw2e.com/

Full-scale treatment plants are maintained using empirical correlations between physicochemical and operational parameters and reactor performance. A thorough study of microbial populations as a function of environmental conditions and how they influence performance is essential for enhancing the stability of process performance and providing crucial recommendations for diagnosis and prognosis. In the end, only bioprocess efficiency matters, regardless of whether the culture is mixed or pure. Due to the complexity of the several phases involved in full-scale treatment, (depending on the type of nutrient removal, the type of product recovery, aerobic or anoxic) maintaining a pure culture on a large scale is exceedingly challenging. But it doesn't imply it is not doable. It is doable if we use distinct bioreactors for each part of the phases. By fine-tuning the physicochemical and operational parameters based on the metabolic route of the particular community, we may maintain the dominant type of a certain community and its clones of interest by suppressing other microbial consortiums. This can ultimately increase the expense of the process.