How to Knock Out GSK3 Alpha in Zebrafish Using CRISPR?

31 January 2025 0 2K Report

I am new to CRISPR and working on a project related to glucose tolerance and insulin resistance in zebrafish. I want to knock out the gsk3α (Glycogen Synthase Kinase 3 Alpha) gene but am unfamiliar with the process. Could anyone guide me on:

How to select the best target sites for gsk3α knockout in zebrafish?

Recommended tools or software for designing sgRNA?

Best methods to confirm successful gene disruption?

Since I am a beginner, any detailed protocols, troubleshooting tips, or recommended reading materials would be greatly appreciated. Thank you!

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