How to it is possible to calculate MBC (Microbial biomass Carbon) from PLFA data?

More Muhammad Israr Khan's questions See All

How can we justify the increase in Toatal Microbial PLFA and Total bacterial PLFA (Gram positive and Gram Negative) after cover crop incorporation ?

we cultivated cover crops during winter season which was harvested and was incorporated in to the soil as a green manure one week before the rice cultivation. To check the effect of cover crop...

10 November 2015 220 13 View

Which one is more reliable to use in Fluorescent in situ hybridization. fluorescence microscopy or confocal scanning electron microscopy?

I want to check the methnaogenic population dynamics in soil by using fluorescent in situ hybridization, but still i don,t know how to select the microscopy technique which may be both efficient...

31 December 2014 9,198 6 View

I want to check methanogenic archaeal population and diversity by using Fluorescent in situ Hybridization (FISH)?

I need some standard protocol for FISH analysis of soil samples, I want to check methanogenic archaeal population and diversity by using Fluorescent in situ Hybridization (FISH)?

11 December 2014 6,381 5 View

Can i use Disodium p-nitrophenyl phosphate Hexahydrate instead of Disoium p-nitroheny tetrahydrate?

I want to prepare PNP solution to analyze acid and alkaline phophatase activity in soil, and for p-nitropheny phosphate solution i could not find the Disodium p-nitropheny tetrahydrate

01 January 1970 3,666 2 View

How to quantify carbon deposition on CuZnAl catalyst?

Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...

01 March 2021 1,907 3 View

Why Trivalent chromium start precipitating above PH 5 ? Moreover discuss difference between adsorption of chromium and precipitation of chromium ?

I am a student of MS Environmental Engineering and started my research phase. I am working on metal removal ( chromium iii ) by using activated carbon prepared from the agricultural feedstock. So...

01 March 2021 5,475 2 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

J. C. Tarafdar

Yes, it is possible to calculate. The bacterial cell numbers derived from bacterial PLFA concentration was an average of 1.17 x 109 per gram dry soil. On the other hand 1.40 x 10-8 nmol bacterial PLFA per cell equal to 2.0x10-5 microgram bacterial C per cell.

Muhammad Israr Khan

Thank you Dr Saab! Appreciate your comments.