How to isolate RNA without dissolution of polymer scaffolds?

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Is there a problem with my RNA pellet?

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Strugglling with m6A dot blot any suugesstion ?

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RNA Extraction Using Hot Borate Method No Longer Working?

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E.coli contamination in human RNA seq data ?

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RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

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What is the acceptable p-value cutoff for GO enrichment analysis ?

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Mario Grossi

If it is ok, trypsinizing to detach cells from scaffolds and then RNA isolation?

Sean Seltzer

Mario gave a good solution, but If you're worried about the effect of trypsinization on gene expression, then I suggest you lyse your cells in a buffer containing rnase inhibitors and then proceed to isolate RNA from the lysate using Trizol LS which is good for isolating RNA from solutions.