How to isolate/purify/characterize a conjugate between an antibody and a fluorescent polymer

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Does anybody have a recommendation for a good stabilizer (other than BSA) for PerCP-Cy5.5 tandem labeled CD antibodies for flow cytometry?

Stabilizer refers to compound that helps to increases mainly photostability of tandem-labelled antibodies

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MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

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MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

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When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

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fatal error

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fatal error

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fatal error

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flexibility of polymers chain

effect of double bond on the flexibility of polymers

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Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

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Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

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How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

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Michael G. Weller

I would start with a protein A separation to get rid of all unbound polymer. All other steps are highly dependent on the polymer you used and the fluorescent dye, which is attached. In general, it is advisable to characterize the two compounds separately, before they are conjugated.

Tanneke den Blaauwen

Protein A colum is a good idea if that is not possible for some reason gelfiltration column or spin down at 300.000 x g