I want to isolate bacteria from dental caries, identify and culture them. What should I do? Please help.
the following pdf will give u a detailed procedure of processing the dental caries for ur experimental studies
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Biochemical characterization and 16S rDNA sequencing can help to identify any bacteria.....you can proceed for biochemical characterization by kit (Himedia or any other) and for 16S sequencing then using blast, you can identify them....you can also use selective media for these pathogens..
Culture samples from dental caries in Mitis-Salivarius Agar Medium. Pick specific colonies and proceed for biochemical characterization.
This is a selective and differential medium for many Streptococci and Enterococcus faecalis. Gram positive cocci in chains have to be subcultured in BHI broth and than submitted to biochemical characterization including sugar fermentation tests and looking at the pH after 48 hs of growth in glucose. For Strept. mutans and S. sobrinus you have to use a selective medium with antibiotics and sucrose at a concentration of 5%. Have a nice work. Take a look at Bergey's Manual.
By taking tooth pin Rub the Upper surface of teeth Gently , or between the teeth, Then make a slide of it , & Perform Spirochete Staining
Hello Shadma. In my publications take a look in the paper Stretococci microbiota associated with inicial plaque formation. Also you can use the metod above to collect samples but sterilize the instruments and put assepticaly in a broth cited in the paper cited. Good luck.
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