how to isolate fungi from powdery mildew diseased sample, kindly share the procedure to isolate fungi from powdery mildew sample.
Sampathkumar M R Despite the fact that the powdery mildew pathogen is an obligate parasite (thus, it needs alive plant tissue for growth), I found a few reports about its growth in vitro. There is no pictures or other confirmation of the fact of such growth, but if you want you can try to repeat the reported methods:
Arabi, M. I. E., and M. Jawhar. "The ability of barley powdery mildew to grow in vitro." Journal of Phytopathology 150.6 (2002): 305-307.https://doi.org/10.1046/j.1439-0434.2002.00751.x
Tu M, Cai H, Hua Y, Sun A, Huang H. In vitro culture method of powdery mildew (Oidium heveae Steinmann) of Hevea brasiliensis. African Journal of Biotechnology. 2012;11(68):13167-72.
Amadioha, A. C. (1998). Control of Powdery Mildew in Pepper (Capsicum annumL.) by Leaf Extracts of Papaya (Carica papayaL.). Journal of Herbs, Spices & Medicinal Plants, 6(2), 41–47. doi:10.1300/j044v06n02_05
To isolate fungi from a powdery mildew diseased sample, you can follow these simple steps:
1. Obtain a diseased plant sample and surface sterilize the infected tissues by washing with 70% ethanol and soaking in a solution of 10% bleach for several minutes.
2. Rinse the sterilized sample thoroughly with sterile water to remove the bleach solution.
3. Cut small pieces of infected tissue and place them onto a sterilized nutrient agar medium.
4. Seal the plate and incubate at an appropriate temperature (usually 25°C to 30°C) for several days to allow for fungal growth.
5. Observe the plate for the growth of fungal colonies, which can be identified based on their morphological characteristics.
Once identified, the fungal colonies can be further isolated by transferring a small piece of the colony onto a new agar plate or by sub-culturing the fungi in liquid media.
*Proper sterilization technique is critical for avoiding contamination and ensuring accurate results.
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