The mixture of microbes (cannot use the term bacteria since the consortium is unidentified) is capable of up taking aromatic hydrocarbon as an energy source (clear zone on the plate). Though when I tried to isolate the microbes by plate purification technique I got two distinguishable strains, but neither of them was able to degrade the compound individually or when inoculated together.
Is there any technique with which I can isolate the microbes more effectively? Since, I suspect that there could be more than 2 strains in the consortium.
P.S.: Formation of the clear zone takes place after 30 hours of incubation. I am using hydrocarbon as the only carbon source.
Maybe streak or spread (a liquid culture) of your consortium on a medium containing the aromatic hydrocarbon as the only carbon source?
I am not using any additional carbon source. My media is supplemented with few salts and my aromatic hydrocarbon.
I agree with Dr. Antoun and would like to suggest you to go for the specific biochemical characterizations and further screen it in a media containing aromatic hydrocarbons.
Good luck!
Thank you for the suggestions Dr. Antoun and Dr. Sandilya. Can I do the gram staining of the consortium and then visualize the cells under a microscope?
Of course you should go for morphological characterization..
Dear Rawat,
Please find the papers attached. Hope it may clear your ideas.
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