How to introduce new restriction recognition sites in vector?

More Ruizhi Tang's questions See All

How to find standard axial compressor blade data files ?

I'm looking for standard axial compressor blade row data files for CFX simulation , so could somebody tell me where can I find them ? Like rotor37 , PBS rotor , and something else about them...

13 February 2021 7,745 3 View

How to do ORR test with pine(Which means RDE or RRDE electrode)?

I want to do LSV ,CV test of ORR(Oxygen reduction reaction). But our laboratory don't have the RDE and RRDE electode. And I was wondering if I can do the tests by magnetic stiring or mechanical...

14 January 2021 1,869 2 View

Why fluorescence signal drops during thermal shift assay?

I am performing a TSA to investigate binding of a ligand to the protein by performing a serial dilution of ligand against same conc. of protein (~2uM). Interestingly at low ligand conc the...

02 August 2020 3,167 1 View

What kind of mass movement is this?

While I'm working on a project about landslides on the Tibet Plateau, I encountered many large and valley-filling deposits that seem to be some kind of mass movement but cannot find their source...

30 July 2020 6,134 5 View

Can anyone help me about probability sampling?

Now I am doing my DBA research, the whole population are the public university teachers in one of Chinese 's province, there are 22 universities. and I want 400 samplings, the total population is...

23 February 2020 248 3 View

How to generate nucleotide and amino acid sequence in same figure just like cloning journal sequence?

Hi all, I am writing manuscript for cloning research, I want to ask if there's any software that allow us to generate nucleotide and amino acid sequence in one same figure, I have been doing using...

15 January 2020 6,290 3 View

How to separate ester from carboxylic acid by using chromatography?

I want to separate my target compound, 1, 3-phenylene bis[4-{[4-(pent-4-en-1-yloxy)phenyl]diazenyl}benzoate (ester) from the reactant 4-{[4-(pent-4-ene-1-yloxy) phenyl] diazenyl} benzoic acid. I...

05 January 2020 1,836 10 View

Did someone read the papar about the relationship of creative climate and creative self-efficacy?

What do you think of the relationship of creative climate and creative self-efficacy is? Please share some opinions of yours. And if you have any paper to recommend, I'd be very grateful. Thanks...

20 December 2019 1,951 3 View

How to study the influence ot feedback on creative self efficacy?

I want to study the inflence of feedbcak on creative self-efficacy. And this is an activity of students. They will receive feedback after this activity. But the question is the feedback is given...

16 December 2019 7,526 3 View

Does anybody know about how to develope the SUSTAIN model by USEPA?

we are considering to develope the SUSTAIN model adding some new functions. the officia website on USEPA applied the source code of its interface and simulation engine, but it's an extension tool...

05 September 2019 4,312 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

How to analyse a split-plot design in stata ?

I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...

14 February 2021 7,800 7 View

Can I use a likert survey for criterion sampling in a qualitative study or would that make it mixed methods?

I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...

09 February 2021 8,594 3 View

How to create the context-mechanism-outcome configuration (CMOc) in the realist methodology?

In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...

08 February 2021 2,792 1 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

How do I create a categorical variable, level of exposure, and a dummy dichotomous, yes-no, variable for the Harvard Trauma Questionnaire Part 1?

I am using the Harvard Trauma Questionnaire (HTQ) Trauma Events scale (original version) that has 17 items (17 different traumatic events) with 4 response options, indicating 4 levels of exposure:...

04 February 2021 1,509 3 View

How to report path analysis / SEM with Yuan-Bentler correction from R output?

Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...

04 February 2021 8,993 2 View

Jack Li

PGL3 should not be too big. You can design a pair of primer with your EcoRI and NotI and PCR the whole plasmid out and transform into E. coli. Usually I can get more than 90% colonies with the right sites in. Go to check NEB website for details:

http://www.neb.com/nebecomm/products/productF-541.asp

Ruizhi Tang

Thanks ,Jack Li,NEB is very useful.

Hoda EL-Attar

how to design a pair of primers