I'm doing a metabolic tracing of glucose on 2 conditions in cell lines. One is treated with a drug. While it is more obvious that I can deduce the altered metabolism rate of glucose and it's metabolites by looking at the labeled fraction and pattern, I am not sure if the unlabeled fraction also tells u sth. More specifically, I wonder if the unlabeled fraction can be interpret as the shift of the metabolism profile of the cell in each condition (while the labeled fraction is the shift of metabolism upon receiving glucose)?
The current limitation of my experiments is I do not have time 0 data. I only get 0.5, 1, 2, and 24 hours. I just want to know if I can deduce the "baseline" by looking at the unlabeled fraction.
I also have another group of experiment that is traced with labeled glutamine. I want to know if the interpretation will be the same.
Mr. Wong,
You can carry out these research tasks quantitatively and 3D structurally; furthermore, exactly. Currently, this can be achieved, only, via our innovative own-authored (mine and my co-author's ones according to the authorship shown, below) stochastic dynamic mass spectrometric theory and model equations applicable to complex mixtures in condensed phases at pg.(mL)-1 concentration level of analytes, yielding to /r/=0.99997-1.
Please, consider the following works and reference sections, therein:
[1] Analytical Chemistry Letters, 10 (2020) 703-721; Stochastic Dynamic Mass Spectrometric Approach to Quantify Reserpine in Solution; Bojidarka Ivanova, Michael Spiteller;
[2] Steroids, 164 (2020) 108750 Stochastic dynamic mass spectrometric quantification of steroids in mixture — Part II; Bojidarka Ivanova, Michael Spiteller;
[3] B. Ivanova, M. Spiteller, Mass spectrometric stochastic dynamic 3D structural analysis of mixture of steroids in solution – Experimental and theoretical study, Steroids 181 (2022) 109001:
[https://www.sciencedirect.com/science/article/abs/pii/S0039128X22000393]; and
[4] Journal of Molecular Structure, 1260 (2022) 132701, Stochastic dynamic quantitative and 3D structural matrix assisted laser desorption/ionization mass spectrometric analyses of mixture of nucleosides; Bojidarka Ivanova, Michael Spiteller, respectively.