How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Upto how much %age of phase can be visible as peak in XRD?

When we do XRD analysis, high intensity peaks are obtained. But there are some very small peaks too which seem to be negligible. Some phases are confirmed from SAED pattern on the basis of...

01 January 2021 3,727 2 View

How do I determine the d spacing and ultimately the hkl values of monocrystalline materials from the SAED pattern?

Below I've attached the image I got from SAED of my material and I'm trying to determine the d spacing and ultimately its hkl values. I used Fiji image J TEM suite for my other samples which were...

25 November 2020 6,392 3 View

Is it possible to get higher order of diffraction (n>1) in HRTEM?

The SAED pattern, we obtained from HRTEM shows the interplanar spacing (d) that corresponds to a higher 2theta value than the Powder crystal X-ray diffraction pattern for the same organic...

02 November 2020 8,073 3 View

What does this SAED image of nanoparticle sample signify?

I couldn't make any inference from this image. please help

30 August 2020 8,442 4 View

How to convert a SAED pattern into a 2D plot like XRD profiles (using GMS3)?

I try to use the line profile, but it just intersects with a few spots in the whole SAED pattern. Is there any way to sum all the spot intensity as a function of distance in reciprocal space?

12 July 2020 7,178 2 View

How to interpret the SAED pattern?

Exact interpretation to analyse the core shell nanostructure materials

09 October 2019 6,391 3 View

Is it possible to get amorphous nature from the SAED pattern ( diffused rings) and lattice fringes from the TEM image for the same sample?

For my sample, diffused rings are observed in SAED pattern which is due to the amorphous nature of the sample. In XRD also, amorphous nature is reflected by the presence of broad peaks. But, in...

10 June 2019 7,367 8 View

NBED versus SAED?

I hardly see any published results for electron diffraction done using the nanobeam ED settings. Is there a reason for prefering SAED over NBED?

04 May 2019 8,716 3 View

How to improve the dispersion of magnetic nanoparticles (such as manganites)?

Improving the dispersion of manganites nanoparticles, it is convinient to obtain the particle size or SAED patterns by SEM or other measurements.

03 April 2019 9,931 10 View

I have a SAED pattern of the hybrid nanostructure (metal and semiconductor) but I am not sure what information should I get from this image?

This SAED pattern is generated for a metal semiconductor hybrid nanostructure. I can see it's not single crystalline but I am not sure how to index the planes? Could you please help?

01 February 2019 2,413 6 View