Hello! I just finished up a co-IP experiment in which I pulled through for CDK1 and probed for Cyclin B1. I did both direct and indirect methods for each sample. During my co-IP, some samples got CDK1 antibody and others got mIgG antibody. When I probe for Cyclin B1, I am seeing it in all lanes, including the ones with mIgG.

I want to only see the Cyclin B1 in my CDK1 lanes. Any thoughts?

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