Peptides in question short (10-15aa) but mainly hydrophilic.
I am considering the the following:
1.Maleimide activated lipid (dope-peg(500-2000-mal) conjugation with Cys-Peptide. The product will then be mixed with lipids in chloroform, and dried lipid film will be rehidrayed and extruded.
2. Maleimide activated lipid (dope-peg(500-2000-mal first included into liposomes as above, followed by conjugation with a peptide.
1 is preferred as method, but I am worried about finding solvent system where both the lipid and a peptide can be dissolved (DMF maybe?)
3. Use lipidation modification option upon protein synthesis. This would be the best option, but I don't know if I can expect the product to be soluble in chloroform to allow liposome preparation by lipid film hydration.
What method would you use and why?
Maybe you have a better suggestion?
Thank you
Try green methods described in the following literature:
1.
Rasti, B., Jinap, S., Mozafari, M. R., & Abd-Manap, M. Y. (2014). Optimization on preparation condition of polyunsaturated fatty acids nanoliposome prepared by Mozafari method. Journal of liposome research, 24(2), 99-105.
2.
Colas, J. C., Shi, W., Rao, V. M., Omri, A., Mozafari, M. R., & Singh, H. (2007). Microscopical investigations of nisin-loaded nanoliposomes prepared by Mozafari method and their bacterial targeting. Micron, 38(8), 841-847.
3.
Jahanfar, S., Gahavami, M., Khosravi-Darani, K., Jahadi, M., & Mozafari, M. R. (2021). Entrapment of Rosemary Extract by Liposomes Formulated by Mozafari Method Without Employing Toxic Solvents or Detergents: Physicochemical Characterization and Optimization. Available at SSRN 3900332.
4.
Mozafari, M. R., Javanmard, R., & Raji, M. (2017). Tocosome: Novel drug delivery system containing phospholipids and tocopheryl phosphates. International journal of pharmaceutics, 528(1-2), 381-382.
5.
Jahadi, M., Khosravi-Darani, K., Ehsani, M. R., Mozafari, M. R., Saboury, A. A., & Pourhosseini, P. S. (2015). The encapsulation of flavourzyme in nanoliposome by heating method. Journal of food science and technology, 52(4), 2063-2072.
6.
Mozafari, M. R., Reed, C. J., Rostron, C., Kocum, C., & Piskin, E. (2002). Formation and characterisation of non-toxic anionic liposomes for delivery of therapeutic agents to the pulmonary airways. Cellular and Molecular Biology Letters, 7(2), 243-244.
Thank you, Nick Gee and M. R. Mozafari !
M. R. Mozafari , could you please indicate one best free access source or copy the protocol in here?
Dear @oleksiy
Please clarify some details including number of aminoacids in the peptide, the hydrophobic or hydrophilic nature of peptide (regarding aminoacid profile), isoelectric point of peptide, and finally the application of this peptide (as a targeting agent or therapeutics).
Dear Alireza Naderi Sohi ,
I need this liposome recruitment to introduce short (7-15 aa) water-soluble peptides originating from protein cytoplasmic tails into in vitro reconstituted systems. No therapeutic constraints apply in this case.
Thank you and best,
Oleksiy
Dear Oleksiy Kovtun
Have a look at attached files (I am sending you PDF version of some of the papers you requested).
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