What condition does need for synthesis MACl(methykeamonium chloride) by use of the precourse solutions pf MA and HCl?

I used to synthesis MACl by myself and I don't even know that the reaction between the MA and HCl leads to MACl or not? I need complete info about the synthesis.

10 February 2020 9,649 4 View

TCAD for quantum devices?

Can somebody please confirm which tool is recommended for a 3d simulation of quantum devices where accurate computation of eigenstates is required for sub 10nm devices? I think Sentautus device is...

17 December 2019 2,075 6 View

How should I represent input variables for recurrent neural network design?

Dear colleagues, I am trying to design a recurrent neural network to predict patients' length of stay as output. I have different types of data (numeric, categorical, free text, etc..) and I want...

13 February 2019 1,386 4 View

How to use steady state solution to define Time dependent initial condition?

Hi, I'm remodeling a paper, and I need to define a perturbation in my initial conditions to validate my model with the results in paper. Paper has calculated the stationary form of the equation...

07 December 2017 346 3 View

How we can make magneto Graphene without applying the magnetic field

How we can make magneto Graphene without applying the magnetic field?and investigate the optical properties of graphene?

18 June 2017 2,156 1 View

How can I remove protein and other pollution from RNA extraction with trizol?

07 April 2017 1,633 3 View

Hello, I'm looking for the physical activity scale for the elderly questionnaire with scoring, could anyone share a free link with me , Thanks

I have searched on google and pubmed but can't locate the scoring.

08 April 2016 5,989 6 View

What are the basic differences (or advantage and disadvantage) between tunability in the ring resonator based filters by these three methods?

11 January 2016 4,709 3 View

When does the oil and protein content of a seed increase simultaneously?

In most cases, when oil% in a seed increases, protein% decreases. But in some cases, these two materials increase simultaneously. when? why?

24 December 2015 5,383 3 View

Are there any effective microglial marker promoter Cre's other than Cd11bCre?

I am looking for an effective Mouse microglial Cre that can be used to breed with mice expressing global Lox P sites. Using a microglial marker Cre will allow for tissue specific conditional...

10 September 2013 3,795 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

How to dissolve querceitin in HEPES?

I am trying to do the isothermal titration calorimetry study of quercetin. I want to reach a final concentration of 5 mM quercetin in HEPES or MES buffer with

15 February 2021 2,159 3 View

XRPD Quantification - Building a calibration curve of drug excipient?

I am aiming to utilise XRPD to quantify a drug excipient within a drug product and have been having a little trouble building the calibration curve to allow for this quantification up until this...

10 February 2021 6,145 3 View

The best RNA isolation kit for lncRNA RNA-Seq?

Hi, I am preparing a new project on lncRNAs in human cancer cell lines. I scanned through many high-impacted journals to find the best RNA isolation kit that would also isolate small RNAs and that...

09 February 2021 4,180 5 View

Why am I getting such large heats of dilution in my Isothermal Titration Calorimetry Experiment?

I am trying to measure binding of calcium to an expressed protein we are studying and am getting very large heats of dilution for the calcium solution in my control tests. We prepared a 1 M CaCl2...

07 February 2021 7,532 1 View

Does anyone know how to isolation of high-purity myenteric plexus from adult rat gastrointestinal tract for further biochemical investigation?

I would like to isolate myenteric plexus from the rats small intestine and then isolate certains components of ENS (enteric nervous system) by biochemical tests.

04 February 2021 6,068 2 View

What could be the possible causes of DNA degradation after extraction from 4% gel?

Hi everyone, During small RNA library preparation, I run the samples in 4% agerose gel to select the band corresponding to my target. I established beforehand the protocol and it works fine....

02 February 2021 5,008 2 View