Hi Vivek,

I need to find out what system you are using or how your samples are coming to you, so I hope this answer helps. To improve RNA quality and achieve a good RIN (RNA Integrity Number), prioritize proper sample collection and handling by quickly freezing tissues in liquid nitrogen, using RNA stabilization solutions like RNAlater, thoroughly homogenizing samples in a chaotropic lysis buffer, and ensuring all labware and solutions are RNase-free; the best assay system for assessing RNA quality is typically an Agilent Bioanalyzer, which uses a proprietary algorithm to calculate the RIN based on the electrophoretic profile of the RNA sample on a microfluidic chip.

Adding 1:200 β-mercaptoethanol or DTT to your lysis buffer can help protect your RNA by inhibiting RNases that may be released during cell lysis. This step prevents RNA degradation, ensuring better RNA integrity for downstream applications

John Pirro , Anton Lennikov

Thank you for the answer. I am encapsulating the mammalian cells in alginate beads by extrusion process. I am using RNA later for stabilization, and bead beater for the alginate beads disruption to take the cells out. In the previous attempts, I have used lysis buffer from Qiagen Mini kit but that didn't work.

I am adding β-mercaptoethanol in 1:100 ratio in TRI reagent before bead beater disruption to take the cells out.