How to improve photo alignment FFS mode display ? What’s the key point ? Post baking ⁉️ PI film ?

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Abnormal data in in-vitro drug release study?

I'm doing in-vitro drug release study using dialysis bag method and collecting samples from the external medium at several time points, subjecting to UV-Vis spectrophotometry. But my absorbance...

14 July 2024 6,829 2 View

Are my cells contaminated with mycoplasma?

I suspect my cells are contaminated with mycoplasma. I fixed the cells with 4% PFA and stained them with DAPI. Below is the image I obtained. I don't observe the typical small, rounded DAPI foci...

11 July 2024 7,786 3 View

Black dots like contamination in my cell cultures. What are they?

There are black dots that seem like contamination in my corneal endothelial cell cultures. Some of the cells appear unhealthy and are enlarged. The black dots are more prevalent around these...

11 July 2024 4,829 0 View

What is the most simple language and character in the world?

Dear colleagues, As well known, we can deliver messages in different languages and characters, including Chinese, English, Latin, binary and decimal codes (if with a converter) and many other...

08 July 2024 5,422 13 View

Trouble shooting on mediation with survival regression and linear regression?

I did linear regression of X (independent variable) to M (Mediator) then I used survival regression to fit X to Y (dependent variable) With these questions: a. HOW to correctly do a mediation...

17 June 2024 1,478 6 View

Why is the blue and white spot screen negative for all white spots?

The protein was expressed using the insect cell system, and the recombinant plasmid was successfully constructed as verified by sequencing, and the recombinant plasmid was transformed into DH10...

13 June 2024 2,590 4 View

Can anyone recommend high-quality GSDMD antibodies for immunofluorescent or immunohistochemical staining in mice?

11 June 2024 6,948 1 View

How to perform the anaysis of taxonomic on raw reads from the mixed sample?

Hi, I am very new to Bioinformatics. Recently, I have the project that aims to perform taxonomic anlysis on raw reads from mixed samples taken from environment. For example, we perfromed NGS...

09 June 2024 6,537 3 View

Weird bubble-like spots on the Western blot membrane?

Hi, I have a question regarding western blot. I performed total protein statin after trasfering membrane. The result was totally fine before (Figure 1). However, there were strange bubble-like...

30 May 2024 8,045 3 View

Is the Gamma function completely monotone within the interval (0,1)?

I am wondering if the Gamma function is completely monotone within the interval (0,1). I use Mathematica to plot the derivatives of Gamma function up to 10th order of derivatives and the results...

29 May 2024 2,846 3 View

What procedure should I follow for current controlled PPy film electrochemical deposition on ITO?

For example, I perform passivation process before electropolymerization of Py for adhesion to titanium surface. Is a similar or different procedure required for ITO? If necessary, I would be glad...

06 August 2024 3,176 0 View

How to increase citation in Research Gate?

How to enhanced h-index in Research Gate?

04 August 2024 3,368 4 View

H-index issues?

Hi Sir/Madam, I would like to know why my h-index is stuck to 15 despite the number of new publications added. Could you explain me how marks are granted. Best, Daniel

28 July 2024 2,607 2 View

How can I better use imageJ to get good result on calculating surface area of a film accurately?

I have images of film formed on the bottom of a 50mL beaker, and I have been trying to figure out the surface area. I could easily use features on imageJ to do that, but I want to know which area...

23 July 2024 4,461 1 View

BD Cell Viability Kit - TO evaporation?

Hello, I'm conducting flow cytometry experiments using the BD Cell Viability Kit and I've noticed that the TO solution ends much faster than the PI solution. I store the solutions according to...

22 July 2024 2,257 0 View

Why can't I detect the plasmon resonance angle with water?

I am trying to measure the plasmon resonance angle of gold film and pure water using the Kretschmann configuration and a 633nm laser. Without flowing water over the gold, I can detect a clear...

10 July 2024 4,719 3 View

I want to ask why the poly aniline film does not stick to the ITO type substrate knowing that I used the electrodeposition method?

In order to make a polyaniline film, I used the electrodeposition method and set all the conditions, but I noticed that the polymerization process is happening, but it doesn't stick to the...

09 July 2024 2,708 2 View

Refractive index in the W-band (75-110 GHz) of some material (Teflon - PTFE) ?

08 July 2024 9,140 1 View

What is polymer/chemicals can increase the swelling index of a polymeric film?

Polymeric film such as latex rubber substrate or polymer

01 July 2024 8,343 4 View

How can we reduce the porosity of tungsten oxide (WO3) thin films during DC sputtering deposition (target: W, power: 100 W, 75 W, and 50 W)?

We are fabricating a tandem structure where a ~50 nm thick WO3 film is deposited between two metal films using DC sputtering. We use a mixture of Ar and O2 gases at different flow rates while...

01 July 2024 2,686 2 View