I intend to do some research on TNTs. The question is, except directly observing the tunneling nanotubes using the immunofluorescent microscope, is there any reliable marker that can be utilized as a piece of evidence for TNT formation?
Dear Ho Nguyen Tuong many thanks for asking this interesting technical question. For some relevant information about this topic please go through the following potentially useful articles which might help you in your analysis:
First of all, here is a good review article on this topic which has been published Open Access:
Opportunities and Challenges in Tunneling Nanotubes Research: How Far from Clinical Application?
(see attached pdf file)
The following two articles are freely available as public full texts on RG and can be downloaded as pdf files::
Cell Connections by Tunneling Nanotubes: Effects of Mitochondrial Trafficking on Target Cell Metabolism, Homeostasis, and Response to Therapy
Article Cell Connections by Tunneling Nanotubes: Effects of Mitochon...
Tunneling Nanotubes and Gap Junctions–Their Role in Long-Range Intercellular Communication during Development, Health, and Disease Conditions
Article Tunneling Nanotubes and Gap Junctions–Their Role in Long-Ran...
For a useful article on markers to evidence the formation of tunneling nanotubes also please see:
M-Sec: Emerging secrets of tunneling nanotube formation
Article M-Sec: Emerging secrets of tunneling nanotube formation
Good luck with your research and please stay safe and healthy! 👍