Are you a microbiologist? There are several tests you need to conduct in order to know the characteristics (biochemical, morphological, cultural, molecular, physiological) of the organism. To get the methodologies for such tests, try and consult practical microbiology texts such as Cheesebrough's District lab practice, Prescott's lab practice in microbiology and many others. After doing, the tests, record the results and then compare the characteristics with those of known organisms in Bergey's Manual of Determinative or Systematic Bacteriology.

Hai,

First of all you have to do Gram staining to identify your organism is positive or negative, afterwards you have to do several Biochemical tests (iMViC tests), after these test you have to combining all the results and you get idea about the genus level, if you want to identify species level you may go with 16rRNA sequencing and phylogeny analysis.

 if you're in a hurry,, you may go with 16rRNA sequencing and phylogeny analysis, your identification will be ready!!

Mass Spec is a quick and easy way to get a size/mass based result