How to i ensure my inoculum has sufficient microbial population to utilize my substrate(rice husk),in anaerobic digestion process?

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Rety Setyawaty

Dear Raja Murugan

I will try to answer your question. This research is first research. The first, I assume that the content of population is same as a pure culture so the content of inoculum same for pure culture. Maybe after I get the result from this research, I will repair for the content of inoculum.

Thanks and regards,

Samsher Singh

You have to optimize the inoculum level by adding the different concentration of cells starting from billion and dilute upto ten thousands level (vary 1 log: by diluting 1:10). After the fermentation you may assess the particular constituent in substrate. For example if you are producing amylase you may assess the starch level and compare with starting level. In this way you may assess the utilization.

Other way is that you may regrow the microbial biomass and assess the productivity of your desired product or enzyme. By comparing the two you may get an idea that second time most probably you would get lower product.