How to give end-of-day far-red treatment to arabidopsis? What is the method for calculating R/FR ratio?

More Manjul Singh's questions See All

Can anyone explain what exactly and ethically "Plagiarism" means...how strictly the issue is being followed in recent scenario by the journal system?

is there any percentage similarity index to judge plagiarism?

10 November 2014 1,983 1 View

Is it necessary to perform two way ANOVA or is a students T test sufficient?

I have some experimental results with my samples having different mutants and different treatment. I was performing student t test for analyzing whether the data is significant or not. I want to...

31 December 2013 819 7 View

How to overcome drooping of inflorescence in healthy Arabidopsis plants?

I am facing some serious issue related to death of Arabidopsis plants due to inflorescence drooping. I have used Bavistin and Chlorpyrifos for curtailing this infection. I can't see any aphids...

07 August 2013 6,159 4 View

Controlling for pupil light reflex when analyzing pupil size time course?

I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...

10 August 2024 3,229 0 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

T. Dornbusch

The cleanest would be to measure the spectral distribution (using a spectrometer) and compute the R/FR ratio as you like. Publish the spectra and the computation in your paper and anybody can reproduce your conditions.

Manjul Singh

Thanks Dornbusch.... can you suggest some economical spectrometer