I am adding here the image, actually i have to calculate the total value of the variable stored in the .tif tile for the boundary closed by blue lines, i have several these type of polygons, here in the .tif file Evapotranspiration data is stored, i have to calculate the total value of evapotranspiration for the area closed by blue boundary (Sub-basin), every area is consists of lots of pixels of .tif file. How it can be done in GIS or any other image processing software?