I have tried to get nanoemulsion pictures but I can not get the picture of nanoemulsion. Can anyone kindly suggest how to get an SEM picture.
Dear Titus Sobisch Sir,
Thank you for your kind reply. When I use phase contrast microscope, I could able to get fine oil droplet globular shape picture. So I have confirmed my sample has globular shape structure. But If i want to get the picture through SEM i could not get properly and moreover, I dont know the sample preparation methodology specifically for the nanoemulsion. Kindly, can you help me to solve this problem?
You need cryo-SEM and prepare specimens by freeze fracture.
Environmental SEM will not give you usable results: you can see only surface of liquid, not what is inside.
However, you may try to use ESEM with highest possible voltage (30 kV?); may be depth of beam penetration will be enough to observe nanospheres. BSE may work better that GSE, but it all depends on your specimens, and quite possible you will not get good results.
Dear Vladimir Dusevich,
Thank you so much for your kind information...
Hi! Have you tried STEM? I am also looking for a method that is more full proof.
Dear Alicia Botes , I have used TEM for getting the Nanoemulsion image and published in RSC Advances. If you want kindly refer to this article "Fabrication of a nutrient delivery system of docosahexaenoic acid nanoemulsions via high energy techniques". Link: Article Fabrication of a nutrient delivery system of docosahexaenoic...
If you need further assistance from me, please let me know.
Best Wishes,
P. Karthik.
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