1) Dear Colleagues; now I want to fractionate the crude hydro-alcoholic extract using different solvents like (water, methanol, chloroform, butanol and ethyl acetate) by using Soxhlet apparatus. So how to perform, and from which solvents I am going to start and continued till the end!
2) Is there any importance if I am using methanol for crude extraction, and further used this solvent again for fractionation of the crude extract?