I am working with a protein and trying to resolve it on Blue-Native PAGE. Can somebody suggest be a recipe for it?

I have been trying to perform Blue-Native PAGE using procedure from doi:10.1038/nprot.2006.62. However, the stacking gel does not solidifies even after two hours, at room temperature. Can...

04 April 2019 4,777 2 View

I am planning for Native PAGE for a protein with pI 8.5 - 8.7. What should be the minimum difference of pH of buffer to avoid electrode switching?

The Gel has pH 8.9. and the pH of loading dye is 6.67. What should be the pH of running buffer to allow smooth running of my protein into the gel. I prepared buffer of pH 8.49 but I was unable to...

25 March 2019 9,877 6 View

Any suggestion what's going on?

I submitted a manuscript on a novel and interesting finding on July 30, 2018 to a reputed journal. Following editorial review it went to peer-review. It is over 8 months, no decision yet. What...

24 March 2019 9,737 2 View

I have used monohydrate citric acid in water for synthesis of graphene quantum dots instead of anhydrous, i have got unsatisfactory results ?

is this is because i have used monohydrate instead of anhydrous? or any other reason plz any one help

03 February 2018 1,596 1 View

How to convert monohydrate citric acid into anhydrous citric ?

someone mentioned that heat it at 78 degree Celsius, is this is the only thing to do or something else? plz anyone tell me the proper method.

03 February 2018 9,039 3 View

How can I isolate a particular region in my periodic domain and gather the data inside that small region using ansys fluent?

How can i isolate a particular region in my periodic domain and gather the data inside that small region using ansys fluent?

01 December 2015 6,180 1 View

What are the advantages growth of GaN on graphene in optoelectronics?

I would like to write my research plan for my PhD on this topic.

21 April 2015 4,301 3 View

Is it compulsory/mandatory to examine the mediating effect in the conceptual framework that was analyzed utilizing AMOS?

Increased value of invertebrates - Mediating variable

08 April 2015 2,834 5 View

How is oil content estimated in mustard seed? Can anyone provide me a protocol regarding this?

I want to estimate the percent of oil present in mustard seed.

24 March 2015 2,233 7 View

What is the minimum sample size needed to conduct the SEM analysis utilizing AMOS software?

05 February 2015 9,648 7 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Glass fiber content in a gypsum board ?

Any idea on how to get the volumic ratio of glass fiber in a gypsum board ? Thank you for your help !

01 March 2021 2,501 3 View

Concrete compressive strength databases?

I am looking for datasets similar to this one: http://archive.ics.uci.edu/ml/datasets/Concrete+Compressive+Strength I am looking for concretes with different types of aggregates (Recycled,...

27 February 2021 6,801 3 View

How to properly calculate the fluid solid heat transfer between polypropylene matrix and glass fiber in a porous medium?

I'm building a cfx model of thermoplastic pultrusion process on ansys and i have a doubt on how is the proper way to set this parameter in a porous medium

24 February 2021 1,915 1 View

How to properly calculate Interfacial area density in a porous domain?which are possible value for glass fiber?

I'm doing a cfx simulation of a multiphase flow in pultrusion and i set the Interfacial Area density in the heat exchange of a porous medium in this way IAD=Total fiber lateral surface of fiber...

23 February 2021 6,043 1 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

Monte Carlo simulation?

Hi, i am conducting a Monte Carlo simulation for my new distribution but the MSE values are increasing while the sample size increase, does any experience this issue before and what should i do...

22 February 2021 3,680 4 View

How can I measure the thickness of thin layers deposited on textured glass susbtrates?

I want to measure the thickness of aluminum oxide and titanium oxide layers deposited on textured glass substates. The layer thicknesses are typically below 100nm.

21 February 2021 5,465 4 View