If I have a .gff file or a .faa file, how can I use this information to understand what the average length of all the sequences are.
Or is there a program to visualize the distribution of sequence lengths?
Many NGS quality analysis tools will do this, the PRINSEQ web service is probably the easiest way using a .faa file.
https://edwards.sdsu.edu/cgi-bin/prinseq/prinseq.cgi
Upload your .faa file and run just basic statistics and you'll get some numeric stats and graphical distributions.
Agree with Joseph
in linux you can simply do:
awk '{/>/&&++a||b+=length()}END{print b/a}' file.fa
Cheers
I see many different studies using RAG1 or 2 knock out mice. I know that this means that lymphocytes will not be fully mature since. T cells will not have a TCR and b cells will not make...
10 November 2019 7,646 3 View
I am wondering if there is a way to get a nucleotide file of just the conserved regions of the 16S ribosomal RNA gene in bacteria to use for finding and obtaining 16S gene sequences from a...
08 September 2018 8,530 3 View
I have a list of taxa names at the family and genus level and their relative abundances. Is it possible to make a phylogenetic tree out with just the names and also add in a marker to demonstrate...
08 September 2018 2,177 3 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...
07 August 2024 269 3 View
Program: g_mmpbsa, version 2024.1 Source file: extrn_apbs.cxx (line 152) Fatal error: Failed to execute command: $APBS pybYcUWA.in --output-file=pybYcUW.out
07 August 2024 6,066 0 View