Forgive my ignorance...
I have the DNA sequence for the LCVR and HCVR of Adalimumab.
I need a resource to find the DNA sequence LC and HC Constant regions specifically Ig G1(HC) and Kappa (LC).
I tried NIH Nucleotide search but no luck.
If someone could point me in the right direction that would be much appreciated.
Thank you for your time.
Hi My Dear,
The drug is expressed from a vector containing sequences for the variable regions obtained via phage display library methodology and sequences containing the human IgG constant domains.
You can access the HC constant region here: https://www.ncbi.nlm.nih.gov/nuccore/AJ294730.1
LC constant region here: https://www.ncbi.nlm.nih.gov/nuccore/AJ294735.1
Immunoglobulins have a characteristic Ig-fold structure consisting of two anti-parallel β-pleated sheets stacked on top of each other and held together by a disulphide bond. The CDR is in the loops joining the β-strands (see, for example, fig. 11.3 of my "Fundamentals of protein structure and function"). Loops in general tolerate sequence variations much better than strands or helices and also have the structural flexibility required for binding.
Identifying the loops amidst the β-strands is a standard task that any structural prediction software can do.
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