I would like to test a biological method of screening vegetables/fruits for the presence of pesticide residue. Since a biological agent is used, the extraction solvent should be non-toxic.
Since most of the organic solvents are more or less toxic to biological agents, commonly used solvents (like ACN, ethyl acetate) to extract pesticide residues from fruits and vegetables may not solve your purpose. The only non-toxic solvent i.e. water can be tried, though most of the pesticides may not get extracted by water.
You can certainly take help from SGS India Bvt. Ltd. I assume their head office is in Chennai. Our lab takes help of their expertise. They also have a multiresidue screening procedure for a large variety of pesticides to be analyzed.
please check this attached file
http://www.eurchembull.com/index.php/ECB/article/viewFile/23/pdf
I suggest to take small sample fo fruit and vegetabled e. g. and follow QuEChERS sample preparation. Your sample is fruit/vegetable. But for the extraction you will make a sampling and you will use just small portion of homogenized sample. For the extraction use only 10g. Why it must be non-toxic? For what is used bological agent?
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