Dear Khaled,

You may use exactly the same products as for Human species, except that you will probably have to adapt a little the volumes of various reagents because of the difference in blood count between Man and farm animals. Genomic DNA is indeed extracted from leukocytes present in venous blood.

Supposing that you're lloking for manual protocols of extraction, you may try kits from Macherey Nagel (the Nucleospin collection; http://www.mn-net.com/Portals/8/attachments/Redakteure_Bio/Protocols/Genomic%20DNA/UM_gDNABlood.pdf). The equivalent kit from GE Healthcare (formerly Amersham), which works pretty much the same is Nucleon BACC2 (https://www.gelifesciences.com/gehcls_images/GELS/Related%20Content/Files/1314774443672/litdocRPN8501PL_Rev_B_11-2007_web_20110831102735.pdf). I have seen that GE Healthcare proposes the Nucleon BACC3 kit, but I never used it.

Qiagen sells also kits for extraction that are quite robust, but I never tested them personally.

I haven't tested the above kit in farm animals but in dogs, and it worked fine. If you want to adjust the protocol, you may ask the echnical support, as they will probably have already done the test for you.

I hope that it helps.

regards, Sébastien

Dear Sébastian,

Thank you very much for your reply, I'll see what protocol I should follow.

Thank you again

best regards.

You're Welcome Khaled. Feel free to ask if I can help.

Best regards,

Sébastien

Dear Khaled,

I agree with Sebastian, MN kits work well, I used them for years to extract DNA from blood, swabs, tissues, molts and others. I also used Wizard kit from Promega to extract DNA from goat and cattle blood for sequencing purposes and I had no problem with quantity and quality of DNA.

and one more point: Qiagen Kit for blood and tissues also works well, and its really good for hair bulbs and bones. However I prefer MN since you can buy each buffer separately in bigger packing, so you have no unused columns. The same you can do with Wizard kit. This is more practical when you need to extract DNA from huge number of samples and you want to modify protocol.

Dear Beata,

I thank you for your precious help. can' I use blood after thawing in case I can not directly use the fresh blood?

Best regards

In my case sequencing works well also when I used DNA extracted from 10-years old cattle blood samples thawed few times.

Dear Beata,

I confirm your experience on thawed samples, even though it is not highly recommended, because you may have bas suprises with the amount of DNA extracted. In my lab, we keep a small volume of blood that may be used for extraction following forensic procedures or automated extraction. Yet, we also spot doublons of blood samples on FTA paper, which is very convenient for storage.

Khaled, another protocol is required for extraction from FTA paper, though.

Best regards,

Sébastien

Dear Sebastian

I agree old blood samples are not perfect samples ;) , but sometimes (as in my case) its the only possibility to analyze archival data of individuals that exists only in a form of old blood or semen samples (e.g. extincted maternal or paternal lines).

We also use FTA cards especially when we need samples without EDTA or heparin for more sensitive applications than standard sequencing or microsatellites analysis. (simply tubes are cheeper than cards).

Regards

bp

Dear Prusak,

Indeed, the use of fresh blood of livestock is difficult, levies on land, especially in large areas must be in EDTA tubes and frozen thereafter prior to extraction

thanks

Use EDTA tubes for whole blood collection and then Qiagen extraction kits.

Hi

what protocol is required for extraction from FTA paper,

I use MN Tissue and blood xs kit