In my research, we want to fabricate scaffold of collagen type I and nano-hydroxyapatite for bone regeneration. For that, we want to extract collagen type I from pig skin (the tissue might be changed). From reviews I've learned that salting out is the best way to extract collagen type I for it's not require hydrolysis.
Does salting out is the best way? If you have any protocol for extraction, please send me. I'll be thankful for insights and tips for the procedure.
Thank you
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3162468/
Tissue Eng Part C Methods. 2011 Sep; 17(9): 879–885. Published online 2011 May 31.
Ultrarapid Purification of Collagen Type I for Tissue Engineering Applications
Christina A. Pacak, Ph.D.,📷1,,2 Jared M. Powers, B.S.,1 and Douglas B. Cowan, Ph.D
Check if the authors are on RG.
The salting-out method is so far the best method to extract the collagen .
S. Béatrice Marianne Ewalds-Kvist Saurav Ranjan Mohapatra Thank you so much.
For all the visitors, Christina A. Pacak had published another good article for collagen extraction:
Article An Improved Method for the Preparation of Type I Collagen From Skin