The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

Is there any general protocol for immunohistology staining?

I have some fixed slides of tissue and need to do some staining, but I am not sure where to start. There are many protocols online. Any suggestions?

17 February 2021 3,062 4 View

How can i figure out whether nanoparticles in my synthesis crosslinked or not?

i prepare protein nanoparticles. in one of my synthesis i use trifle amount of crosslinker and the nanoparticles stick in the bottom of plate. how can i understand that nanoparticles become...

03 December 2020 559 8 View

How to examine the substitution of ligands bound to protein during the MD simulation?

I have simulated a complex ( A protein docked with two ligands), and the ligands are bind to two different protein sites. Still, from the start of the simulation, ligands are positioned in other...

27 November 2020 7,848 4 View

How to change the atom numbers of a residue in a pdb file?

I am trying to dock 2 proteins in HADDOCK server, but it repeatedly shows the following Error for the second pdb file: " Error in PDB file. Your PDB contains multiple residues with number 1 in...

25 November 2020 4,753 1 View

Is it possible that formazan Crystal formation took more than four hours ?

Hello dear scientists: When I treated the Pc12 cell line with H2O2, after 2h the cells seemed to be dead. I didn't observe crystals, so I didn't add DMSO and left it with mtt in the...

20 October 2020 1,106 3 View

How can i detecte the fluorescence and non-fluorescence complex?

hello kind regards I study the structure and activity of the digestive enzymes. The enzyme I studied contains 5 amino acids tryptophan. In one of the studies, I study the effects of a food dye on...

04 October 2020 8,563 3 View

How to increase the number of nanoparticles in fe-sem?

I produce a protein nanoparticle complex,but in fe-sem, I see a few number of nanoparticles,maybe 2 or 3 ,however the color of suspension solution is quite milky and this is a sign of...

29 August 2020 8,812 5 View

Why would a negative control have apoptosis in the Annexin V-PI Flow Cytometry?

06 August 2020 3,627 22 View

How can we induce gastroesophageal reflux disease in rats?

Gastroesophageal reflux disease (GERD) occurs when stomach acid frequently flows back into the tube connecting your mouth and stomach (esophagus). This backwash (acid reflux) can irritate the...

14 June 2020 145 8 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View