From among all reducing sugars, how can I calculate the quantity of sucrose? Is there any particular method for the estimation of sucrose? After incubation of sugarcane juice for 24 hrs, sucrose is converted into dextran and gets reduced. How one can quantify the quantity of sucrose at every stage of process?
i am not sure but you can go through Cole's method for sugar estimation. attachment may be useful to you.
thank you chintan for information. I will have a look on it.
by hdrolyzing it using hydrochloric acid then estimatin the resultant monosaccharide using Benedict's quantitative or DNS method and you should first estimate reducing sugar in sample because in addition to sucrose there is also glucose in cane then substract the concentrtion of total reducing after hydrolysis from concentration of reducing before hydrolysis you will get the concentration of reducing result from sucrose hydrolysis then multiply in Moris factor (0.95) to get concentration of sucrose
thank you Thoria donia for the solution. I will definitly go thru it.
Estimate the reducing sugar by DNS method and also do estimation of total sugars by anthrone method the difference will give sucrose content in sugar cane.
You can use HPLC for the analysis. You will need RI detector. Also YSI sugar analyser is an instrument which provide direct quantification of reducing sugars. This works on enzyme immobilized membranes.
Schamer' has reported on a rapid and accurate method for the analysis of sucrose using a gas liquid chromatographic procedure, thus opening the way for routine Sucrose analyses.
Also for analysis part details refer to
SchaMer, K. J. (1976). Preliminary comparison of polarimetric and gas chromatographic methods for the estimation of sucrose in sugar cane mixed juice and molasses - SASTA Proc 50 : 220-223.
If you need only to estimation (not high accuracy ) you can use a refractometer
with the help of some following quantitative analysis, we can estimate sucrose contents:
Reducing sugar test by titration method
Brix % by Refrectrometer
Pol % by polarimeter
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