As the presence of dextran is very low in sugarcane, how one can judge that the dextranase has degraded the dextran present in the sugarcane juice? How quickly can we find out whether the enzyme is actually acting on the dextran present in the juice? Is there any method to find out the reaction? Is there any physical change occur in the cane juice after reaction with dextranase?
Sara Catone
theorically, hidrolysis of dextran should translate in a chenage of polarity and densityof the sugarcane juice. anyway, there are some methods to follow the hydrolisis based on the measurement of the reducing sugar formed (i.e. Anon (2002b) Dextranase activity assay procedure. Bio-Cat Technical Method.-quite old, but you can modify it on your requirements).
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