I want to estimate starch in any starchy food (like flour, rice, potato etc). I want to quantify the amylose and amylopectin separately. If anyone have a written estimation protocol please provide me.
I recommend the protocol by K. Brunt et al., Starch 50 (1998) 413-419, suitable for the determination of starch in a wide range of foodstuffs. This method is based on complete enzymatic hydrolysis of starch to glucose. The method has been validated in an interlaboratory study.