Dear colleague:

Digestibility is not a great issue, in vitro gas production or nylon bag OM and N degradability techniques are very suitable. The estimation of ME is more complicate because, among others aspects, it can be influence by the called antinutritive factors, that, as tannins, could improve or affect ME.

Depending on the scientific problem you are working to solve, it will be the choice. Any way you can check:

https://revistas.reduc.edu.cu/index.php/rpa/article/view/2822

best regards,

Redimio

Dear Prof. Redimio Manuel Pedraza Olivera ,

Thank you for your answer and also for the reference, I will take time to read it. It is certainly interesting work and looks useful in order to understand the relationship between variables.

We were originally hoping to conducted en saco digestibility work, however, that is unfortunately no longer possible (though we are retaining samples for the future, just in case).

Your comments relating to tannins and ME are certainly a reason we are taking caution in how we approach this.

Once again, many thanks,

Andrew.

0k, colleague. there is a paper of Pagella et al about the use of a novel approach to do in sacco in animal that will be slaugther;

Development of an intra-ruminal nylon bag technique for feed evaluationwhich does not require the use of fistulated animals. 2010. dvances in Animal Biosciences: Food, Feed, Energy and Fibrefrom Land - A Vision for 2020, Proceedings of the British Society of AnimalScience and the Agricultural Research Forum, Annual Conference, April 2010,p. 26. Cambridge, UK: Cambridge University Press.

Also, you can check several papers on use of PVP and PEG at in vitro gas production to asses the effect o tannins on digestibility.

best regards,

Redimio