How can I estimate fructose in my sample by spectrophotometer method like carbohydrates estimation. Is there any spectrophotometer method for it.
2/24/22
Dear Karisma,
Here is an enzymatic method that works.
www.megazyme.com/d-mannose-d-fructose-d-glucose-assay-kit
I have used it. It is easy to do and works very well. I hope this helps you.
Bill Colonna
Iowa State University, Ames, Iowa, USA [email protected]
Hello, I'm conducting a beta galactosidase staining assay for my fibroblasts using X-Gal. I dissolve X-Gal (stock 50mg/ml prepared in DMSO) in the staining buffer at a final concentration of...
25 March 2024 8,799 3 View
The absence of Ocean Island Basalts (OIBs) in the Archean rock records is commonly attributed to the higher melting degrees associated with mantle plumes. However, this explanation raises...
14 March 2024 2,195 1 View
Mostly bacteria living inside plant roots.
14 March 2024 4,602 0 View
What is the correct way to calculate the BSSE correction for a molecule having 3 fragments? Please see the attached image for details. I know the values are pretty close but still I would love to...
25 October 2023 3,990 1 View
Can I use DIN and PO4 in the given formula : The trophic index (TRIX) used in the present work has been given by the following formulation (Vollenweideret al.1998): TRIX = [log(Chla∙ (D%)O2 ∙ TN ∙...
26 September 2023 432 1 View
Many think that, because of the vastness of the oceans, the continents were underwater throughout the Hadean and much of the Archean periods. It wasn't until the Mesoarchean that continents began...
19 September 2023 6,875 0 View
I am trying to quantify 5mC and 5hmC using Epimark 5mC and 5hmC kit from NEB. I did the end-point PCR after glucosylation and digestion with MspI and HpaII. It didnt generate a a yes or no answer,...
06 September 2023 6,221 0 View
How can the number of carbon quantum dots (CQDs) internalized per cell be calculated using the "IntraCell V2" plugin in ImageJ?
01 September 2023 419 0 View
The EPMA analysis for FeO for native metal (Iron) is coming as 110, 124, 114 (wt%). What could be the possible reason?
20 July 2023 2,583 0 View
I need to calculate oxygen exchange current density using polarization resistance.
07 June 2023 9,237 2 View
it's important to know that we are using the inactive elements of Cs, Sr, Co
25 July 2024 1,553 2 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
I have conducted a study on the fructose, glucose, and sucrose compound content in tomato flesh extracts. The obtained peak areas aligned with the chromatogram graph. However, the concentrations...
19 July 2024 449 2 View
I have conducted a survey on the "effect of vacuum drying conditions on carbohydrate content of angelica.S". The result show that, when increasing the vacuum degree, the carbohydrate content first...
13 July 2024 592 1 View
zzz
02 July 2024 918 2 View
The line appears always in the penultimate run. We try to decrease the volume of the solvent system, wich is composed of butanol:acetic acid:water (2:1:1) (76 mL) but the line remains. Below I...
24 June 2024 9,829 3 View
spectrophotometric determination of iron and copper
04 June 2024 1,720 0 View
Hi everyone, has anyone else experienced an 'Error 8008' with their NanoDrop 3300 (image attached), on a laptop which it previously worked fine on? I have tried uninstalling the software and...
02 June 2024 9,025 3 View
Previously I have used GlyProt to add carbohydrate chains with my modelled protein, but the server is not working now. Is there any alternative tool for modeling glycosylated protein?
01 June 2024 894 1 View
I want to determine the conjugated drug to IgG ratio by nanodrop. I know the extinction coefficient of IgG and drug by M-1cm-1 but all the unit in the nandrop program is based on g/ml and so on...
15 May 2024 9,097 4 View