How can I estimate fructose in my sample by spectrophotometer method like carbohydrates estimation. Is there any spectrophotometer method for it.
2/24/22
Dear Karisma,
Here is an enzymatic method that works.
www.megazyme.com/d-mannose-d-fructose-d-glucose-assay-kit
I have used it. It is easy to do and works very well. I hope this helps you.
Bill Colonna
Iowa State University, Ames, Iowa, USA [email protected]
I tried to fit the frequency vs Dielectric constant and f vs Dielectric Loss graph with HN equation. I got different Tau (relaxation time) values from the fittings of both graphs. What I have to...
12 January 2021 8,467 6 View
In most of the research paper people used both neutral and alkaline comet assay to determine the level of DNA damage either single stranded or double stranded but the exact principle of both the...
02 August 2020 2,193 2 View
In many countries, the children belong to remote villages and do not have facility of computer or Android mobile and internet connectivity. Do you think, the countries will be able provide online...
17 May 2020 2,739 16 View
Using TURBOMOLE, I am studying a mechanism of Heterolytic vs Homolytic cleavage. When the bond breaks, there are three possibilities a) Heterolytic Cleavage (RHF, singlet) b) Homolytic Cleavage...
15 October 2019 4,214 5 View
Need methods for isolation and characterization of bio-active peptides in milk with proper protocols for the same.
12 December 2018 3,818 2 View
I want to obtain the vegetation index map for a date which lies in between the 16 day composite MODIS vegetation indices. Ex- Let's say the MODIS (both terra and aqua) has 16 day composite images...
01 September 2018 9,489 6 View
Logistic regression, SVM, andom forest etc
24 July 2018 1,019 4 View
I have come across a lot of journals with different solutions. But I am not sure which one can be used. Please suggest.
19 July 2018 1,903 1 View
In detail, I performed flexural test of some composite specimen. I got higher flexural strength with high modulus. But after doing some modification in main material, I got enhanced flexural...
15 April 2018 4,826 7 View
I am looking into monocytes and macrophage in murine model (C57Bl/6) of a disease.
22 March 2018 959 2 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...
01 March 2021 6,999 3 View
I measure the wavelength of material in a spectrophotometer and it peaks at 275 and its absorbance is 100. I mix it with an adsorbent and it adsorbs the first material, but when I measure the...
16 February 2021 7,515 5 View
rats were given daily dexamethasone in drinking water corresponding to 0.5 mg/kg or 2 mg/kg. These rats are simultaneously fed 25% fat, 3% salt in diet and 10% fructose in drinking water but...
15 February 2021 7,634 2 View
I'd like to know if anyone can provide me with a protocol for traditional catalase enzymatic assay in a 96 well microplate, for cell line and/or C.elegans spectrophotometric measurements. Thanks!
11 February 2021 7,273 3 View
I know colorimetry has been the standard way by way of measuring the concentration of a particular compound (solute) in a colored solution (solvent or measure quantities of a particular compound...
08 February 2021 2,673 1 View
Hi there, I would like to determine the unknown concentration of human insulin in my sample during crystallisation (the concentration range is roughly 0.5 -2 mg/ml, whereas the initial...
06 February 2021 9,370 7 View
Hi guys. I make RNA by in vitro transcription and do cell transfection afterwards. I analyze RNA using Nanodrop and Bioanalyzer. But I found the concentration of RNA measured by these two machines...
05 February 2021 292 3 View
I have prepared gold nanoparticles using the citrate reduction method in the lab. I need to characterize them using UV-Vis and DLS, by how much should I dilute my sample for using it in the...
26 January 2021 530 5 View
Prosopis juliflora is considered a noxious weed but its eradication is very costly inhivitive if no impossible ! We have very strong openion that it's spread could be chacked only if it's pods are...
25 January 2021 4,433 7 View