I am going to add carbon source as sodium acetate and Nitrogen source as ammonium sulfate in basal media for nitrification experiments.
I would like to estimate carbon/nitrogen ratio in basal media.
I need a method to estimate carbon nitrogen ratio in basal media.
I am finding most of references towards soil carbon nitrogen ratio estimation.
Kindly please provide any reference with reference to carbon nitrogen ratio estimation in liquid media.
Thank you
For more info. regarding you question, please have a look at this article.
Effect of C/N Ratio and Media Optimization through Response Surface Methodology on Simultaneous Productions of Intra- and Extracellular Inulinase and Invertase from Aspergillus niger ATCC 20611
· Estimation of nitrogen-to-carbon ratios of organics and carbon materials at the submicrometer scale
Julien Alleon, Sylvain Bernard, Laurent Remusat, François Robert
Abstract: Precisely estimating nitrogen-to-carbon (N/C) ratio of carbonaceous materials at the submicrometer scale is a challenge in both natural and material sciences. Following recent attempts reported in the literature, the present paper discusses two methods of quantification of the N/C ratio or organics and carbon materials at the submicrometer scale using nanoscale secondary ion mass spectrometry (NanoSIMS) and X-ray absorption spectroscopy (XAS). The present data highlight the need to use at least two standards to build a calibration line in order to precisely and accurately (± 0.009 – 95 % confidence level) estimate N/C values of unknown carbon samples using NanoSIMS. As shown here using a set of reference compounds, STXM-based XAS allows directly estimating N/C ratios of organics without using any standard with errors as low as 0.007.
The present contribution presents careful examinations of two methods recently reported in the literature for precise quantification of the N/C atomic ratio of organics at the submicrometer scale based on nanoscale secondary ion mass spectrometry (NanoSIMS) measurements and X-ray absorption spectroscopy (XAS) data collected using scanning transmission X-ray microscopy (STXM). From results obtained on a set of organic reference compounds, the present contribution discusses the validity, precision, advantages and limitations of these two methods.
Tuning of the Carbon-to-Nitrogen Ratio for the ... - MDPI
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PDFNov 10, 2017 - C/N ratio in E. coli fermentations has further been demonstrated for heterologous gene expression [30] ... Batch fermentations in minimal medium were performed in ... Figure 3. The fermentation profiles with different C/N ratios during growth in bioreactors with. Figure 3 ... Licensee MDPI, Basel, Switzerland.by M Ginésy - 2017
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