At what time points should pro-inflammatory cytokines levels be tested in a mouse model of Prototheca algae infection?

I'm working on establishing an murine infection model of the Prototheca algae. Not much is known about the dynamics of the infection, nor the induced immune response. I'd like to use the...

19 October 2020 2,908 2 View

Why I observe the Si peak after analyzing PEO on EDX?

After putting the PEO coated glass substrate into the oven and subjecting it to a temperature of 180 degrees, it obtains a haze on the glass. A peak Si was observed after EDX analysis. Is there...

24 September 2020 6,335 6 View

Which reference gene would you recommend for qPCR analysis in mouse macrophages and epithelial cells?

Which gene would you recommend as reference in those types of cells? Until now, I was working with HPRT but it's not efficient for me anymore. Thank you for all of the answers!

22 September 2020 615 4 View

Meaning of first derivative dM/dH around magnetization reversal in magnetic nanoparticle systems?

When I calculate first derivative of M versus H curve measured for an ensemble of FM nanoparticles (below they blocking temperature) I obtain a very clear maximum, very close to the so called...

04 May 2020 5,116 4 View

IHC Andy Algorithm, how to use it?

Hello everyone! Probably, this issue was already raised, but I would really appreciate online help from someone who didi it. So - could anyone help me with fiji, Andy's algorithm? I try to run it...

26 January 2020 2,249 1 View

Can Alltech OA 1000 Organic acids column be used to analyse solutions in solvents such as DMSO, THF, butanol or ethanol?

I would like to study the dehydration of carbohydrates to HMF in other solvents than water, however I am not too sure if the HPLC column that we have is appropriate for analysing this type of...

03 April 2019 4,022 5 View

How to obtain correctly DC demagnetisation curve (for nanoparticles)?

How can I obtain correctly DC demagnetisation curve (for nanoparticles)? How should I specifically perform the measurements: a) Do I bring to saturation my sample once and then apply increasingly...

20 January 2019 8,441 3 View

How I know that structure is porous from XRD measurement?

In XRD results for porous boron-carbon samples I've got a huge signal (11 000 arb. units) for small angle at 2theta=2.7. I know that this signal can indicate the preaense of small particles or...

14 July 2018 9,366 15 View

How can I distinguish S. cerevisiae var. boulardii from other S. cerevisiae strains (preferrably by molecular methods)?

Does anybody know which molecular typing methods would be the best for checking if the strain of S. cerevisiae is boulardii or not? I can't determine microsatellites polymorphism due to a lack of...

02 April 2018 3,857 2 View

What is proper gating strategy for LIVEDEAD stained population in order to exclude dead cells from analysis?

Hi! I would like to discriminate dead cells from my population using LIVEDEAD fixable dye. In principle it stains amine residues in both live and dead cells, but because of cell membrane...

28 October 2017 5,109 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

No title

01 March 2021 3,905 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

Sample size determination for non-parametric tests?

How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...

24 February 2021 166 6 View

How to determine a priori sample size for t-test with unequal means?

I need to conduct a a priori sample size determinations for t-test with unequal means, but I don't know the sizes of the groups nor the ratio of both groups... Can I do a sample size determination...

24 February 2021 8,289 3 View

What are alternatives to factor analysis for a small sample?

My sample size is too small to conduct a factor analysis. I created my own psychometric scale for measuring Effectiveness of Support, which contains 38 items (4 sub-scales) Im really struggling...

23 February 2021 9,287 12 View

Missing Data in Pretest and Post test- Experimental Research design?

Hello all, I am analyzing some pretest and post-test data that were given to evaluate knowledge of targeted vocabulary before and after the intervention. Pretest and post-test are identical and...

23 February 2021 9,400 7 View