Which is the best mitochondrial potential dye for primary cells?

Hi! I am wondering if someone can recommend me a good dye for measuring mitochondrial potential. I have used TMRE with good results in the past, but I have heard also about Mitoview. I would use...

12 January 2021 4,200 4 View

Why neuromyths (misconceptions of brain and cognitive function) are still present in education?

We seek reflections of professionals and academics of education, for the installation of neuro myths in education in Latin America

26 December 2019 2,254 3 View

Can you include a focus group with only one participant in your data analysis?

We are analyzing data from a qualitative research study in which we conducted focus groups. However, for one focus group, only one person showed up. We went ahead and did the focus group, but can...

04 November 2019 4,335 11 View

How long after my last allergen challenge can I measure Mouse Mast cell protease-1 (mMCP-1)?

Hi: I have lung tissues I can homogenize to measure different cytokines. I am also interested in measuring some Mast cells proteases if possible(specifically mMCP-1). The problem is, lung tissues...

15 October 2019 9,291 2 View

What dilution should I use for my mice lung homogenates to perform a BCA total protein?

Hi community: I was wondering if you could give me more tips on the following. I am looking at cytokines levels of lungs exposed to an allergen. Specifically, I am looking at intracellular...

25 September 2019 6,686 7 View

How to add a Journal to the available ones when adding and article to ResearchGate?

11 December 2018 7,043 0 View

Do you know any community-level dataset with at least 20 years since the first survey?

I'm looking for community-level datasets older than 20 years, at the landscape spatial-scale (i.e. multiple survey points in space), for any taxa or region. If you know any dataset with these...

26 December 2017 4,467 1 View

What kind of thickening agents are used before compression of Epoxy-based SMC composites?

Unsaturated Polyester and Phenolic resins are usually thickened with alkali metal oxides and alkaline earth metal oxides before compression in sheet molding compound. For epoxy resins do these...

04 September 2017 4,348 1 View

How can i improve standard curves values in RT q-PCR miRNA assay?

We are using TaqMan Advanced kit, the efficiency in most of our standard curves is 150%, with low slopes (-2,6) but high R2 (>0.99), how can I improve results?Has changing the dilutions...

21 July 2016 1,948 6 View

Can anyone recommend any published studies on the physiological effects of saline injections insects?

I've been trying to find published studies about the physiological effects of saline injections in insects. So far, I have only found one paper (Jarosz, J (1988) Cytobios 53:19-29) that suggests...

10 November 2014 3,860 6 View

How two melting peaks in DSC of polypropylene can be merged into one crystallization peak?

The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...

03 March 2021 5,857 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

What information can be had from raman at higher wavenumber?

i have recorded raman spectra of strontium oxide thin film. the spectra were recorded in the range 400 to 4000 cm-1. i have reference to peaks upto 1000cm-1. but i couldn't find literature for...

26 February 2021 2,499 1 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

How can I find a Raman spectrum wavenumber corresponding to what compound?

I have several Raman spectra with very distinct peaks at certain wavenumbers, such as 277, 359, 466, 583, 623, 686, and 795cm-1. How can I identify them to what compound(s)? The RRUFF and others...

24 February 2021 363 3 View

Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View

Why the CHi^2 is close to zero with a bad fitting but the Rp and Rwp are still high?

I am refining my XRD data using in FullProf program. The intensity of the modulated X-ray peaks is almost flat. I tried to adjust the peak intensities but unsuccessful. What is the reason? I guess...

23 February 2021 2,304 4 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View