Mr. Aurum,

Currently, there is only one method, which is capable of exact quantifying analytes directly in solution by mass spectrometry (MS). It appears, only method for highly accurate, precise, sensitive; and in particular, higly selective analysis among the analytical instrumentations, as well.

It is our own-authored (to me and my co-author's contribution according to the authorship shown in the papers, below) development based on stochastiv dynamics. Our model equations appear applicable to analyte concentration levels pg.(mL)-1, thus, yielding to coefficients of linear correlation /r/ = 0.99997-1.

Please, consider our method in references [1,2]. It is applicable universally, in context ionization mass spectrometric methods; in addition to, chromatographic ones used to hyphenated MS methods.

[1] Stochastic Dynamic Mass Spectrometric Approach to Quantify Reserpine in Solution; Bojidarka Ivanova, Michael Spiteller Analytical Chemistry Letters, 10 (2020) 703-721; Received 13 Oct 2020, Accepted 16 Dec 2020, Published online: 28 Jan 2021 Download citation https://doi.org/10.1080/22297928.2020.1865834

[2] Steroids, 164 (2020) 108750 Stochastic dynamic mass spectrometric quantification of steroids in mixture — Part II; Bojidarka Ivanova, Michael Spiteller

If you have any questions, regarding, its application to lipidomics, then, please, ask them, herein. They shall be addressed, accordingly.

Please look at the following below links which may help you in your analysis:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7243740/pdf/fonc-10-00717.pdf

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3278153/pdf/nihms339713.pdf

https://www.nature.com/articles/s41597-021-00894-y.pdf

Thanks

Kate Wolfer thank you for your detailed answer. I am working in lipidomics. Currently, we have developed methods using MRM mode taking the transition of Q1 and Q3 from the available literature. Especially the MRM transition of the lipid species which may be found in plants, not animal/human.

However, the issue is that when we want to run numerous samples and multiple injections, it may cause prolonged analysis time which may probably cause lipid degradation in the vial.

The problem with making the transition from the authentic standard is that there are many lipid species that don't have commercial standards available.

While my purpose is to conduct comprehensive lipidomic profiling for all possible detected species from the known lipid groups.

Do you have any other comments or suggestions? Thanks